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Journal of the Marine Biological Association of India
Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation
The milt of goldspot mullet, Liza parsia (Hamilton-Buchanan) was diluted with four extenders containing 10% DMSO. Samples were collected at four critical steps of cryopreservation procedure. Percentage of intact spermatozoa and cryoinjuries at each step was recorded. Extender V2E in seawater base +lo% DMSO appeared to be the best cryodiluent as the sperms exhibited least structural changes during the cryopreservation protocol. Chao's extender +lO% DMSO was the next preferred cryodiluent. The suitability of various extenders that accorded protection to injuries was evaluated on the basis of electron microscopic images.
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