Journal of the Marine Biological Association of India

Volume 45 Issue 1

Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation

Sandhya Sukumaran, D. Noble, A. Gopalakrishnan and N. K. Sanil

The milt of goldspot mullet, Liza parsia (Hamilton-Buchanan) was diluted with four extenders containing 10% DMSO. Samples were collected at four critical steps of cryopreservation procedure. Percentage of intact spermatozoa and cryoinjuries at each step was recorded. Extender V2E in seawater base +lo% DMSO appeared to be the best cryodiluent as the sperms exhibited least structural changes during the cryopreservation protocol. Chao's extender +lO% DMSO was the next preferred cryodiluent. The suitability of various extenders that accorded protection to injuries was evaluated on the basis of electron microscopic images.

Date : 30-06-2003