Journal of the Marine Biological Association of India

Antibacterial activity of marine-derived Sutcliffiella horikoshii KSV4 isolated from Rushikonda water source, Vishakhapatnam

Volume 66 Issue 2

Dugyala Yogitha Bala, K. Shanthi Kumari and B. Keerthana Goud

doi:10.6024/jmbai.2024.66.2.2508-02

Abstract

Marine bacteria have emerged as prominent sources of novel pharmaceutical agents produced through fermentation processes to meet global demands for antibiotics in recent years. Marine bacteria Sutcliffiella horikoshii, found in marine and soil ecosystems, has been
found to produce a potent neurotoxin, Tetrodotoxin (TTX). This study demonstrates the isolate KSV4 of S. horikoshii, from Rushikonda Beach, exhibiting remarkable antibacterial properties against clinically relevant test bacteria using the Kirby-Bauer agar-well diffusion method. Thin-layer chromatography-mediated bioautography associated with Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed bioactive compounds in the crude extract of KSV4. Additionally, molecular identification confirmed KSV4, suggesting applications in environmental remediation and pharmaceuticals. Overall, this study underscores the potential of maritime ecosystems as a promising source of novel antibiotics, contributing to combating antibiotic resistance. Further research is required to corroborate these findings against a broader range of pathogens and elucidate the bacterial isolate’s therapeutic potential, mechanism of action and biotechnological applications.

Keywords

Antibacterial efficacy, kirby-Bauer disc diffusion method, Sutcliffiella horikoshii, Thin-layer chromatography (TLC)-bioautography, marine bacteria, Dodecane

Date : 17-12-2024