Journal of the Marine Biological Association of India

Antimicrobial activity of the crude peptide extracts of flower tail shrimp, Metapenaeus dobsoni

Volume 65 Issue 2

M. V. Anju, P. P. Adarsh, K. Archana, Ajith Antony and C. R. Reshma

10.6024/jmbai.2023.65.2.2380-05

Abstract

Antimicrobial peptides (AMPs) are widely distributed in various organisms, including plants and mammals. The present study was undertaken to isolate and purify antimicrobial peptides from Metapenaeus dobsoni (Md), Flower tail shrimp. The crude peptides were tested for antibacterial activity against 12 pathogens by disc-diffusion assay. The remarkable antibacterial activity could be observed towards the 11 pathogens (7-20 mm), and three were selected for further assays. For purification, crude peptide extract was subjected to solid phase extraction and cation exchange chromatography. The fractions Md5-3, Md40-1, Md80-3 showed the highest activity towards Staphylococcus aureus, Md5-3, Md40-4, Md40-6, Md80-1 against Vibrio alginolyticus and Md5-3, Md40-6, Md80-1 against Bacillus cereus. M. dobsoni could be a promising source of bioactive peptides. This basal study suggests that M. dobsoni is a source of antimicrobial peptides (AMP), which could be applied to aquaculture fields as therapeutic agents.

Keywords

Antimicrobial peptides, Metapenaeus dobsoni, innate immunity, bacterial pathogens, shrimps

Date : 15-11-2023