Journal of the Marine Biological Association of India

Probiotic activity and antibiotic resistance pattern of microbes isolated from shrimp ponds

Volume 49 Issue 2

Shani John* and Jamila Patterson

Abstract

Bacterial strains from shrimp pond sediment were isolated and identified. The microbes which showed the enzymatic hydrolysis were tested for their antagonistic activity against the fish and human bacterial pathogens. The antibiotic resistance pattern of four fish pathogens and six human pathogens were tested against twelve commercially available antibiotics. Vibrio 7 and Bacillus 1 and 2 spp. showed good antagonistic activity against the disease causing fish pathogens than the human pathogens. The Multiple Antibiotic Resistance Pattern of the isolated microbes was evaluated. Vibrio sp.1 and Pseudomonas sp. showed multiple drug resistance to three-seven of the ten antibiotics tested. The resistance pattern of the microbes may be due to the repeated use of the same drug to control bacterial diseases. The Pseudomonas sp. which showed multiple drug resistance was analyzed for the plasmid and the resistant gene was found in the 3000kb region

Keywords

Enzymatic hydrolysis, antagonistic activity, antibiotic resistance, MAR index, plasmid isolation.

Date : 22-07-2008