Journal of the Marine Biological Association of India

Volume 58 Issue 2

Longfin snake eel, Pisodonophis cancrivorus (Richardson, 1848), as a potential source of antimicrobial peptides


K. Archana, Naveen Sathyan, E. R. Chaithanya, Ambika Gopalakrishnan and Rosamma Philip

Antimicrobial peptides are small, cationic, amphipathic and evolutionarily conserved molecules of the innate immune system of eukaryotes, which they use to wade off a wide range of microbes. The present study was carried out to evaluate longfin snake eel, pisodonophis cancrivorus as a potential source of antimicrobial peptides. The crude peptide extract was obtained by modified acetic acid-acetone precipitation method and its activity was detected by disc diffusion assay. The crude peptide extract was subjected to solid phase extraction using Sep-pak®C-18 cartridges (Waters, USA). The 40% Sep-pak® fractions eluted was further purified by cation exchange chromatography which yielded 5 fractions. Among the five fractions, Pc40-4 and Pc40-5 exhibited the highest antimicrobial activity and showed inhibition against all tested bacterial strains namely Edwardsiella tarda , Bacillus cereus , Vibrio fluvialis , Vibrio cholera , Aeromonas hydrophila and Staphylococcus aureus . Pc40-3 exhibited remarkable antibacterial activity against the Gram-negative bacteria Aeromonas hydrophila (98%). This is the first report on AMPs from Pisodonophis cancrivorus 


 Antimicrobial peptides, Pisodonophis cancrivorus , antimicrobial activity, peptide extract, teleost fish.


Date : 10-01-2017