Occurrence of actinobacteria species in mangrove sediments of the Gaderu River of Gautami- Godavari estuarine system was studied during May 2007. The actinobacteria cultures were grown on czapeks agar, tyrosine agar, kuster agar, oatmeal agar and glucose-aspargine agar media. Twenty two strains of actinobacteria were isolated from the mangrove sediments. Of these isolates, fourteen isolates belonged to the genus Streptomyces; three isolates to Streptoverticillium, and four isolates to Micromonospora. The genus Streptomyces was represented by three species viz., S. parvulus, S. flavoviridis and S. lusitanus. The distribution of actinobacteria species is compared with similar studies elsewhere in India.