The effect of probiotics supplementation with feed on the production of giant tiger prawn, Penaeus monodon, in pond culture was studied. Four ponds (area ranging from 527 to 1196 sq. m) were stocked with P. monodon PL-20 @ 10 nos./ sq m to study the effect of probiotics on production performance. In two ponds the commercial feed without probiotics (i.e. control) and in other two ponds same feed supplemented with probiotics containing Lactobacillus spp. and Saccharomyces sererevisae were given to the shrimps. In all the ponds, tidal water was used for water exchange for 3-5 consecutive days per fortnight when turbidity and dissolved oxygen were around 20 cm. and 5.2 mgll respectively. During culture period, water temperature (⁰C), DO (ppm), pH, alkalinity (ppm) and salinity (ppt) in all the ponds were in the range of 27.5-36.9, 5.2 -1 1.2, 7.22-9.69, 92-164 and 8-15, respectively No aeration was given during culture period. At the end of 120 days of culture it was found that total biomass yield (kg/ha) in probiotic supplemented group was higher (1880.15±71.85) than that of non-supplemented group (1489.65k146.05). Feed conversion ratio (FCR) in probiotic supplemented and non supplemented ponds were 1.33+0.04 and 1.35+0.03, respectively. Survivability (%) of shrimps was also higher in probiotic supplemented pond (71.17±2.24). So, it can be concluded that supplementation of probiotic with feed has beneficial effect in improving survivability and yield of this species in pond culture