Investigations were made on the ovarian maturation of eyestalk ablated Fenneropenaeus merguiensis maintained under different environmental conditions. The results obtained indicated no signs of maturation when females of size smaller were ablated. Larger ablated females showed a delay in maturation during the colder months in contrast to those ablated during the warmer months. The study suggests that a system incorporating unilateral eye stalk ablation, high salinity, good water quality and optimum temperature are sufficient to induce maturation in captivity in females above the length of first maturity.